Resumo
Introdução: Exames laboratoriais fazem parte da pesquisa científica em Educação Física, e tem se expandido, especialmente, na avaliação de biomarcadores metabólicos, hormonais, inflamatórios e hematológicos. Esses marcadores permitem analisar respostas e adaptações ao exercício físico, além de interações psicofisiológicas. No entanto, a confiabilidade dos resultados depende do controle adequado em todas as fases da coleta de material: pré-analítica, analítica e pós-analítica, sendo a primeira a principal fonte de variação e possíveis falhas.
Objetivo: Discutir os principais fatores associados aos erros pré-analíticos em pesquisas na área da Educação Física e suas implicações na interpretação de biomarcadores relacionados ao exercício físico.
Conclusão: Erros pré-analíticos podem ocorrer devido à falta de padronização das condições dos participantes, bem como a falhas na coleta, identificação e processamento das amostras. Em estudos de campo, esses desafios tendem a ser ampliados, podendo comprometer a integridade das amostras e a confiabilidade dos resultados. A fim de prevenir e/ou minimizar a ocorrência desses problemas, recomenda-se a adoção de protocolos padronizados e o treinamento técnico adequado da equipe, pois, são fundamentais para garantir maior qualidade metodológica nas pesquisas em Educação Física.
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